immunomagnetic negative selection based separation (Miltenyi Biotec)
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Immunomagnetic Negative Selection Based Separation, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 488 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunomagnetic+negative+selection+based+separation/CD4%2B+T+Cell+Isolation+Kit%2C+human/pm33356811-55-0-12
Average 97 stars, based on 488 article reviews
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Article Title: Immunomagnetic separation is a suitable method for electrophysiology and ion channel pharmacology studies on T cells.
Journal: Channels (Austin, Tex.)
doi: 10.1080/19336950.2020.1859753
Figure Legend Snippet: Figure 2. Comparison of viability and purity after the different separation techniques. Viability was determined with 7-AAD staining while purity was expressed as a percentage of CD4+ cells in the living cell population (see Methods). Measurements were made via flow cytometry, subsequently after the separation (values are plotted as mean±SD for the number of independent experiments indicated in the bar). No significant differences were found among the groups for either viability or purity (one-way analysis of variance, p > 0.05). REAlease®: measured after the complete removal of bead-antibody and REAlease® complex, in label-free configuration (see Figure 1), Negative selection: immunomagnetic negative selection, Sorted: positive selection by flow cytometry.
Techniques Used: Comparison, Staining, Flow Cytometry, Selection
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